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phosphate group  (TaKaRa)


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    TaKaRa phosphate group
    Phosphate Group, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1999 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phosphate+group/T4+Polynucleotide+Kinase/10__1186_slash_s10086___026___02265___w-74-18-24
    Average 96 stars, based on 1999 article reviews
    phosphate group - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Purification:

    Article Title: Characterization of the effects of terminators and introns on recombinant gene expression in the basidiomycete Ceriporiopsis subvermispora.
    Article Snippet: ssion in many eukaryotes; however, the functional importance of these elements for controlling gene expression in Agaricomycetes remains unclear.. In this study, the effects of Ceriporiopsis subvermispora terminators and introns on the expression of a recombinant hygromycin B phosphotransferase gene (hph) were characterized.. Using a transient transformation system, we proved that a highly active terminator (e.g., the gpd terminator) is required for the efficient expression of the hph gene.

    Polymerase Chain Reaction:

    Article Title: Characterization of the effects of terminators and introns on recombinant gene expression in the basidiomycete Ceriporiopsis subvermispora.
    Article Snippet: ssion in many eukaryotes; however, the functional importance of these elements for controlling gene expression in Agaricomycetes remains unclear.. In this study, the effects of Ceriporiopsis subvermispora terminators and introns on the expression of a recombinant hygromycin B phosphotransferase gene (hph) were characterized.. Using a transient transformation system, we proved that a highly active terminator (e.g., the gpd terminator) is required for the efficient expression of the hph gene.

    Modification:

    Article Title: Characterization of the effects of terminators and introns on recombinant gene expression in the basidiomycete Ceriporiopsis subvermispora.
    Article Snippet: ssion in many eukaryotes; however, the functional importance of these elements for controlling gene expression in Agaricomycetes remains unclear.. In this study, the effects of Ceriporiopsis subvermispora terminators and introns on the expression of a recombinant hygromycin B phosphotransferase gene (hph) were characterized.. Using a transient transformation system, we proved that a highly active terminator (e.g., the gpd terminator) is required for the efficient expression of the hph gene.



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    Image Search Results


    S1P treatment attenuates radiation-induced ovarian injury in rats. A Mean body weight of rats in each group. B Wet ovarian weight of rats in each group. C Representative HE staining images showing histopathological changes in ovarian tissues from each group. D IHC analysis was conducted to detect the expression of MYS2 in rat ovarian tissues. E Quantification of primordial, primary, secondary, and antral follicles per group. F ELISA-based measurement of proinflammatory cytokines TNF-α and IL-1β in ovarian tissues. Serum levels of FSH, estradiol, and AMH were measured by ELISA. Data are shown as the mean ± SD of n = 10 rats per group, and statistical analyses were performed using two-way analysis of variance (ANOVA) with Tukey’s post-hoc test. *** P < 0.001, # P < 0.05, ### P < 0.001

    Journal: Journal of Ovarian Research

    Article Title: Sphingosine-1-phosphate mitigates radiation-induced ovarian injury in rats by suppressing ferroptosis via activating the Nrf2-GCLC-GPX4 axis

    doi: 10.1186/s13048-026-02192-w

    Figure Lengend Snippet: S1P treatment attenuates radiation-induced ovarian injury in rats. A Mean body weight of rats in each group. B Wet ovarian weight of rats in each group. C Representative HE staining images showing histopathological changes in ovarian tissues from each group. D IHC analysis was conducted to detect the expression of MYS2 in rat ovarian tissues. E Quantification of primordial, primary, secondary, and antral follicles per group. F ELISA-based measurement of proinflammatory cytokines TNF-α and IL-1β in ovarian tissues. Serum levels of FSH, estradiol, and AMH were measured by ELISA. Data are shown as the mean ± SD of n = 10 rats per group, and statistical analyses were performed using two-way analysis of variance (ANOVA) with Tukey’s post-hoc test. *** P < 0.001, # P < 0.05, ### P < 0.001

    Article Snippet: For GCLC inhibition, rats in the IR + S1P + BSO group received L-buthionine-(S, R)-sulfoximine (BSO; 10 mg/kg; Cat. No. HY-100031, MedChemExpress, USA) intraperitoneally on the same schedule as S1P.

    Techniques: H&E Stain, Analysis, Expressing, Enzyme-linked Immunosorbent Assay

    S1P protects rat ovaries from radiation-induced ferroptosis. A Representative TEM images of mitochondrial morphology in ovarian tissues in each group. B Quantification of iron accumulation in ovarian tissues using iron assay kits. C-E Levels of MDA, SOD, and GSH were measured in ovarian tissues using biochemical kits. F Intracellular ROS levels in ovarian tissues were detected using flow cytometry. G JC-1 staining was used to evaluate mitochondrial membrane potential. Data are shown as the mean ± SD of n = 10 rats per group, and statistical analyses were performed using two-way analysis of variance (ANOVA) with Tukey’s post-hoc test. ** P < 0.01, *** P < 0.001, # P < 0.05, ### P < 0.001

    Journal: Journal of Ovarian Research

    Article Title: Sphingosine-1-phosphate mitigates radiation-induced ovarian injury in rats by suppressing ferroptosis via activating the Nrf2-GCLC-GPX4 axis

    doi: 10.1186/s13048-026-02192-w

    Figure Lengend Snippet: S1P protects rat ovaries from radiation-induced ferroptosis. A Representative TEM images of mitochondrial morphology in ovarian tissues in each group. B Quantification of iron accumulation in ovarian tissues using iron assay kits. C-E Levels of MDA, SOD, and GSH were measured in ovarian tissues using biochemical kits. F Intracellular ROS levels in ovarian tissues were detected using flow cytometry. G JC-1 staining was used to evaluate mitochondrial membrane potential. Data are shown as the mean ± SD of n = 10 rats per group, and statistical analyses were performed using two-way analysis of variance (ANOVA) with Tukey’s post-hoc test. ** P < 0.01, *** P < 0.001, # P < 0.05, ### P < 0.001

    Article Snippet: For GCLC inhibition, rats in the IR + S1P + BSO group received L-buthionine-(S, R)-sulfoximine (BSO; 10 mg/kg; Cat. No. HY-100031, MedChemExpress, USA) intraperitoneally on the same schedule as S1P.

    Techniques: Morphology, Iron Assay, Flow Cytometry, Staining, Membrane, Analysis

    S1P activates the NRF2/GCLC pathway following radiation exposure. A Western blot analysis of GCLC, GPX4, TfR1, nuclear p-NRF2, and total NRF2 levels in ovarian tissues at 24 h post-irradiation in each group. B IHC analysis was conducted to detect the expression of GCLC, GPX4, and TfR1 in ovarian tissues in each group. Data are shown as the mean ± SD of n = 10 rats per group, and statistical analyses were performed using two-way analysis of variance (ANOVA) with Tukey’s post-hoc test. *** P < 0.001, ### P < 0.001

    Journal: Journal of Ovarian Research

    Article Title: Sphingosine-1-phosphate mitigates radiation-induced ovarian injury in rats by suppressing ferroptosis via activating the Nrf2-GCLC-GPX4 axis

    doi: 10.1186/s13048-026-02192-w

    Figure Lengend Snippet: S1P activates the NRF2/GCLC pathway following radiation exposure. A Western blot analysis of GCLC, GPX4, TfR1, nuclear p-NRF2, and total NRF2 levels in ovarian tissues at 24 h post-irradiation in each group. B IHC analysis was conducted to detect the expression of GCLC, GPX4, and TfR1 in ovarian tissues in each group. Data are shown as the mean ± SD of n = 10 rats per group, and statistical analyses were performed using two-way analysis of variance (ANOVA) with Tukey’s post-hoc test. *** P < 0.001, ### P < 0.001

    Article Snippet: For GCLC inhibition, rats in the IR + S1P + BSO group received L-buthionine-(S, R)-sulfoximine (BSO; 10 mg/kg; Cat. No. HY-100031, MedChemExpress, USA) intraperitoneally on the same schedule as S1P.

    Techniques: Western Blot, Analysis, Irradiation, Expressing

    GCLC mediates the radioprotective and anti-ferroptotic effects of S1P in rat ovaries. A Western blot was conducted to detect the protein levels of GCLC, GPX4, TfR1, and nuclear p-NRF2 and total NRF2 levels in ovarian tissues in the indicated groups. B IHC staining of GCLC, GPX4, and TfR1 in ovarian tissues of each group. Data are shown as the mean ± SD of n = 10 rats per group, and statistical analyses were performed using two-way analysis of variance (ANOVA) with Tukey’s post-hoc test. *** P < 0.001, ### P < 0.001

    Journal: Journal of Ovarian Research

    Article Title: Sphingosine-1-phosphate mitigates radiation-induced ovarian injury in rats by suppressing ferroptosis via activating the Nrf2-GCLC-GPX4 axis

    doi: 10.1186/s13048-026-02192-w

    Figure Lengend Snippet: GCLC mediates the radioprotective and anti-ferroptotic effects of S1P in rat ovaries. A Western blot was conducted to detect the protein levels of GCLC, GPX4, TfR1, and nuclear p-NRF2 and total NRF2 levels in ovarian tissues in the indicated groups. B IHC staining of GCLC, GPX4, and TfR1 in ovarian tissues of each group. Data are shown as the mean ± SD of n = 10 rats per group, and statistical analyses were performed using two-way analysis of variance (ANOVA) with Tukey’s post-hoc test. *** P < 0.001, ### P < 0.001

    Article Snippet: For GCLC inhibition, rats in the IR + S1P + BSO group received L-buthionine-(S, R)-sulfoximine (BSO; 10 mg/kg; Cat. No. HY-100031, MedChemExpress, USA) intraperitoneally on the same schedule as S1P.

    Techniques: Western Blot, Immunohistochemistry, Analysis

    GCLC inhibition reverses the protective and anti-inflammatory effects of S1P in irradiated ovaries. A Rat body weight and B wet ovarian weight in each treatment group. C HE staining was used to analyze the pathological changes in ovarian tissues in each group. D Quantification of follicle subtypes (primordial, primary, secondary, antral) in each group. (E-F) ELISA quantification of TNF-α and IL-1β in ovarian tissues (G-I) Serum levels of FSH, estradiol, and AMH were determined by ELISA. Data are shown as the mean ± SD of n = 10 rats per group, and statistical analyses were performed using two-way analysis of variance (ANOVA) with Tukey’s post-hoc test. *** P < 0.001, ### P < 0.001

    Journal: Journal of Ovarian Research

    Article Title: Sphingosine-1-phosphate mitigates radiation-induced ovarian injury in rats by suppressing ferroptosis via activating the Nrf2-GCLC-GPX4 axis

    doi: 10.1186/s13048-026-02192-w

    Figure Lengend Snippet: GCLC inhibition reverses the protective and anti-inflammatory effects of S1P in irradiated ovaries. A Rat body weight and B wet ovarian weight in each treatment group. C HE staining was used to analyze the pathological changes in ovarian tissues in each group. D Quantification of follicle subtypes (primordial, primary, secondary, antral) in each group. (E-F) ELISA quantification of TNF-α and IL-1β in ovarian tissues (G-I) Serum levels of FSH, estradiol, and AMH were determined by ELISA. Data are shown as the mean ± SD of n = 10 rats per group, and statistical analyses were performed using two-way analysis of variance (ANOVA) with Tukey’s post-hoc test. *** P < 0.001, ### P < 0.001

    Article Snippet: For GCLC inhibition, rats in the IR + S1P + BSO group received L-buthionine-(S, R)-sulfoximine (BSO; 10 mg/kg; Cat. No. HY-100031, MedChemExpress, USA) intraperitoneally on the same schedule as S1P.

    Techniques: Inhibition, Irradiation, H&E Stain, Enzyme-linked Immunosorbent Assay, Analysis

    S1P alleviates radiation-induced ferroptosis by upregulating GCLC. A Iron accumulation in ovarian tissues was measured using an iron assay kit. B-D Levels of MDA, SOD, and GSH in ovarian tissues were assessed using commercial kits. E Flow cytometry was conducted to detect the levels of ROS in rat ovarian tissues. F The TEM images were used to explore the morphological changes of mitochondria in ovarian tissues in each group. G JC-1 staining was performed to detect the mitochondrial membrane potential in ovarian tissues. Data are shown as the mean ± SD of n = 10 rats per group, and statistical analyses were performed using two-way analysis of variance (ANOVA) with Tukey’s post-hoc test. *** P < 0.001, ### P < 0.001

    Journal: Journal of Ovarian Research

    Article Title: Sphingosine-1-phosphate mitigates radiation-induced ovarian injury in rats by suppressing ferroptosis via activating the Nrf2-GCLC-GPX4 axis

    doi: 10.1186/s13048-026-02192-w

    Figure Lengend Snippet: S1P alleviates radiation-induced ferroptosis by upregulating GCLC. A Iron accumulation in ovarian tissues was measured using an iron assay kit. B-D Levels of MDA, SOD, and GSH in ovarian tissues were assessed using commercial kits. E Flow cytometry was conducted to detect the levels of ROS in rat ovarian tissues. F The TEM images were used to explore the morphological changes of mitochondria in ovarian tissues in each group. G JC-1 staining was performed to detect the mitochondrial membrane potential in ovarian tissues. Data are shown as the mean ± SD of n = 10 rats per group, and statistical analyses were performed using two-way analysis of variance (ANOVA) with Tukey’s post-hoc test. *** P < 0.001, ### P < 0.001

    Article Snippet: For GCLC inhibition, rats in the IR + S1P + BSO group received L-buthionine-(S, R)-sulfoximine (BSO; 10 mg/kg; Cat. No. HY-100031, MedChemExpress, USA) intraperitoneally on the same schedule as S1P.

    Techniques: Iron Assay, Flow Cytometry, Staining, Membrane, Analysis